3-CAT high sensitive ELISA

Enzyme Immunoassay for the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine. Flexible test system for various biological sample types and volumes. Adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are extracted by using a cis-diolspecific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.

NAME: 3-CAT Research ELISA

DESCRIPTION: Research enzyme immunoassay (ELISA) for the quantitative and ultra sensitive determination of Adrenaline (Epinephrine), Noradrenaline (Norepinephrine), and Dopamine in any species and various biological samples

DESIGN: Common extraction of any biological fluid in 48-well macrotiter plates. Acylation in liquid phase. 12x8 break apart wells microtiter plate . 6 Standards. 2 Controls. ready for use. Common reagents for parallel testing. Ultra sensitive ELISA for any species and various biological samples

SAMPLE VOLUME: 1 - 750 µl, all biological fluids

TOTAL ASSAY TIME: Extraction, acylation and enzymatic conversion 4 hours and ELISA overnight

STANDARD RANGE: Adrenaline, Dopamine: 0 / 0.5 - 80 ng/ml; Noradrenaline: 0 / 0.2 - 32 ng/ml

SENSITIVITY: Adrenaline: 10 pg/ml; Noradrenaline: 4 pg/ml; Dopamine: 10 pg/ml (depending on sample volume)

STORAGE: 2 - 8 °C

KIT: 3 x 96 Determinations

ADVANTAGES: Test any biological fluid without any interferences!Determine three analytes with one kit (Extract only once!); 48 duplicate determinations or 96 single; Total assay approximately 4.5 hour; sFlexible protocol for various sample volumes and types