Enzyme Immunoassay for the quantitative determination of free Metanephrine and free Normetanephrine in plasma. First, the plasma proteins are removed by precipitation. After this Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are quantitatively acylated. The subsequent competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. NAME: 2-MET Plasma ELISA Fast TrackDESCRIPTION: Fast Track enzyme immunoassay (ELISA) for the quantitative determination of Free Metanephrine and Free Normetanephrine in plasmaDESIGN: Common sample preparation. Acylation in liquid phase. 6 standards, 2 controls, ready for use.SAMPLE VOLUME: 500 µl plasmaTOTAL ASSAY TIME: Sample preparation 15 min and ELISA overnightSTANDARD RANGE: Metanephrine 0 / 36 - 3 600 pg/mlNormetanephrine 0 / 48 - 4 800 pg/mlSENSITIVITY: Metanephrine: 17 pg/mlNormetanephrine: 23 pg/mlSTORAGE: 2 - 8 °CKIT: 2 x 96 DeterminationsADVANTAGES: Simple sample preparationWide standard ranges. convenient measurement of pathological samples without predilutionSpecific for the biologically relevant L-metanephrineCE MARK: CE Marked
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