Enzyme Immunoassay for the quantitative determination of adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine in plasma and urine. Adrenaline (epinephrine), noradrenaline (norepinephrine), and dopamine are extracted by using a cisdiol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. NAME: 3-CAT ELISA Fast TrackDESCRIPTION: Fast Track enzyme immunoassay (ELISA) for the quantitative determination of of Adrenaline (Epinephrine), Noradrenaline (Norepinephrine), and Dopamine in plasma and urineDESIGN: Extraction and Acylation in 48-well microtiter plates (PATENT EPA 1835 290). 6 standards, 2 controls, ready for use.SAMPLE VOLUME: 10 µl urine, 300 µl plasmaTOTAL ASSAY TIME: Extraction and acylation 65 min and ELISA 3 hSTANDARD RANGE: Adrenaline: 0 / 1 - 200 ng/mlNoradrenaline: 0 / 5 - 1 000 ng/mlDopamine: 0 / 4.5 - 2 000 ng/mlSENSITIVITY: Adrenaline: 0.3 ng/ml urine, 10 pg/ml plasmaNoradrenaline: 1.5 ng/ml urine, 40 pg/ml plasma Dopamine: 4.5 ng/ml urine, 25 pg/ml plasmaSTORAGE: 2 - 8 °CKIT: 3 x 96 DeterminationsADVANTAGES: Total assay approximately 2.5 hoursDetermine all three analytes with one kitNo known interference by medical drugsWide standard ranges. convenient measurement of pathological samples without predilutionHigh correlation with HPLCCE MARK: CE Marked
Academic Discount – Customers performing Academic Research are entitled to a 10% discount on all Coagulation and Biochemistry products. Use Voucher Code ACAD10 at checkout.