2-CAT ELISAFast Track (Adrenaline/Noradrenaline)

2-CAT ELISAFast Track (Adrenaline/Noradrenaline)

Brand:LDN
Product Code:BA E-6500
Price: £619.00

Enzyme Immunoassay for the quantitative determination of adrenaline (epinephrine) and noradrenaline (norepinephrine) in plasma and urine. Adrenaline (epinephrine) and noradrenaline (norepinephrine) are extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. NAME: 2-CAT ELISA Fast TrackDESCRIPTION: Fast Track enzyme immunoassay (ELISA) for the quantitative determination of Adrenaline (Epinephrine) and Noradrenaline (Norepinephrine) in plasma and urineDESIGN: Extraction and Acylation in 48-well microtiter plates (PATENT EPA 1835 290). 6 standards, 2 controls, ready for use.SAMPLE VOLUME: 10 µl urine, 300 µl plasmaTOTAL ASSAY TIME: Extraction and acylation 65 min and ELISA 3 hSTANDARD RANGE: Adrenaline 0 / 1 - 200 ng/mlNoradrenaline 0 / 5 - 1 000 ng/mlSENSITIVITY: Adrenaline: 0.3ng/ml urine, 10 pg/ml plasma; Noradrenaline: 1.5 ng/ml urine, 50 pg/ml plasmaSTORAGE: 2 - 8 °CKIT: 2 x 96 DeterminationsADVANTAGES: Total assay approximately 2.5 hoursDetermine both analytes with one kitNo known interference by medical drugsWide standard ranges. convenient measurement of pathological samples without predilutionHigh correlation with HPLC


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