Enzyme Immunoassay for the quantitative determination of dopamine in plasma and urine. Dopamine is extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.NAME: Dopamine ELISA Fast TrackDESCRIPTION: Fast Track enzyme immunoassay (ELISA) for the quantitative determination of Dopamine in plasma and urineDESIGN: Extraction and Acylation in 48-well microtiter plates (PATENT EPA 1835 290). 6 standards, 2 controls, ready for use.SAMPLE VOLUME: 10 µl urine, 300 µl plasmaTOTAL ASSAY TIME: Extraction and acylation 65 min and ELISA 3 hSTANDARD RANGE: 0 / 4.5 - 2 000 ng/mlSENSITIVITY: 4.5 ng/ml urine, 25 pg/ml plasmaSTORAGE: 2 - 8 °CKIT: 96 DeterminationsADVANTAGES: Total assay approximately 2.5 hoursNo known interference by medical drugsWide standard ranges. convenient measurement of pathological samples without predilutionHigh correlation with HPL
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