Tryptophan ELISA

Enzyme Immunoassay for the quantitative determination of Tryptophan in urine, serum and plasma samples.After extraction and derivatization Tryptophan is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards. NAME: Tryptophan ELISADESCRIPTION: Enzyme immunoassay (ELISA) for the quantitative determination of Tryptophan in urine, plasma, and serumDESIGN: Common sample preparation for all biological fluids. Derivatization in liquid phase. 96-well-reaction plate. 12x8 break apart wells microtiter plate. 6 standards. 2 controls. ready for use.SAMPLE VOLUME: 20 µL urine, plasma or serumTOTAL ASSAY TIME: Sample preparation 2.5 hours and ELISA overnightSTANDARD RANGE: 0/2.5 - 250 µg/mlSENSITIVITY: 1.2µg/mlSTORAGE: 2 - 8 °CKIT: 96 DeterminationsADVANTAGES: Please contact us.CE MARK: CE Marked