Enzyme Immunoassay for the quantitative determination of Histamine in urine and cell culture samples and for the quantitative determination of Total Histamine in whole blood.First, Histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated analyte concentrations in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigenantibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-goat IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.
NAME: Histamine ELISA Fast Track
DESCRIPTION: Fast Track enzyme immunoassay (ELISA) for the quantitative determination of Histamine in Cell Cultures and urine
DESIGN: Common sample preparation for all body fluids. Acylation in liquid phase. 96-well-acylation plate. 12x8 break apart wells microtiter plate. 6 standards. 2 controls. Ready for use.
SAMPLE VOLUME: 20 µl cell culture or urine
TOTAL ASSAY TIME: Sample preparation 15 min and ELISA 1 hour
STANDARD RANGE: 0 / 0.5 - 50 ng/ml
SENSITIVITY: 0.2 ng/ml
STORAGE: 2 - 8 °CKIT: 96 Determinations
ADVANTAGES: Easy handling. direct assay. no extraction
No interference by medical drugs known
Wide standard ranges. convenient measurement of pathological samples without predilution
CE MARK: CE Marked
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