Enzyme Immunoassay for the quantitative determination of Histamine in plasma and urine. In combination with the supplementary kit Histamine Release (for details contact your local supplier), the assay can be used for the measurement of histamine release in heparinized whole blood. In the first part of the procedure, Histamine is quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated analyte concentrations in the standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-goat IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm.
NAME: Histamine ELISA
DESCRIPTION: Enzyme immunoassay (ELISA) for the quantitative determination of Histamine in plasma and urine
DESIGN: Common sample preparation. Acylation in liquid phase. 96-well acylation plate. 12x8 break apart wells microtiter plate. 6 Standards. 2 Controls. ready for use.
SAMPLE VOLUME: 10 µl urine, 25 µl plasma
TOTAL ASSAY TIME: Acylation 1.5 h and ELISA overnight
STANDARD RANGE: 0 / 0.5 - 50 ng/ml
SENSITIVITY: 0.12 ng/ml plasma, 0.3 ng/ml urine
STORAGE: 2 - 8 °CKIT: 96 Determinations
ADVANTAGES: No interference by medical drugs known
Wide standard ranges. convenient measurement of pathological samples without predilution
CE MARK: CE Marked
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