OLIGOBIND APC Inhibitor solution

DESCRIPTION
APC inhibitor solution is intended for the collection of whole blood for further preparation and assaying of activated Protein C (APC) plasma levels. The solution contains reversible active site inhibitors of APC. The inhibitor protects APC from inhibition by plasma protease inhibitors in drawn blood ex vivo.

The solution is intended for research use only.
 
USAGE
Add the APC inhibitor solution to the sodium citrate blood collection tube. The inhibitor has to be diluted 1+200 in the tubes (e.g for
blood collection tube with a fill size of 3 ml add 150 µl inhibitor solution).

EQUIPMENT REQUIRED BUT NOT PROVIDED
Sodium citrate blood collection tubes (0,106 mol/l).

PRESENTATION
APC inhibitor solution 4.4 ml (4x 1.1 ml vials). Suitable for 25x 3ml blood collection tubes.

Recommendation for blood collection
1. Collect blood into Blood Collection Tubes containing sodium citrate and the inhibitor solution:
    - puncture vein by using sterile blood collection system, such as a wing canula with tubing (“butterfly”)
      and appropriate Luer-Lock adapters
    - note: the first blood draw (up to 3 ml) should not be used for APC determination, therefore use
      other blood collection tube (up to 3 ml), e.g. for other analysis than APC determination.
    - then immediately draw blood into APC Blood Collection Tube(s).
    - discard material in case of initially unsuccessful puncture attempt or obviously impaired blood flow
      during sampling.
    - do not puncture the same vein again but preferably chance to other limb for second attempt.
2. Drawn blood should be stored cooled (4 °C) and centrifuged (see step 3) within 4 hours.
3. Centrifuge the blood sample at 2,500 x g for 15 minutes.
4. Plasma should be stored at 2°- 8°C and assayed within 4 hours.
    Alternatively, plasma may be stored at -20°C for up to 6 months.
5. Frozen plasma should be thawed rapidly at 37°C.

Follow the general guidelines for plasma preparation and handling of potentially infectious specimens.

Blood-to-additive variation can cause erroneous results if the tubes are over filled or under filled.

STORAGE AND STABILITY
Store APC Inhibitor solution at 2°-8°C. Solution may be used until the recorded expiration date. Shipment of product does not require cooling during the time of transportation.

Category: Research use only