murine MAb abainst human factor VIII, heavy chain

Description
The monoclonal antibody (clone ESH-5) is directed against human factor VIII:C. Mice were immunized with purified human factor VIII:C cryoprecipitate. Hybridomas were created by fusion of the immunized spleen cells with NS-1 murine myeloma cell lines.  

Properties
The antibody (subclass IgG₁) is directed against the A1 domain of intact heavy chain (functional epitope).
The cross-reactivity was tested via IRMA, in comparison with human plasma (100%): the antibody does not cross react with Pig, Dog, Baboon, and Cat, the antibody does cross-react with Rabbit (212%) and Guinea Pig (204%).

Presentation
Screw capped vial containing 0.5 mg of purified antibody in 50 mM sodium phosphate, 50 mM trisodium citrate, 10% Maltose pH 5.8, 0.01 % ProClin. The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
A. Immunopurification / Immunodepletion
The antibody binds factor VIII:C from plasma when covalently coupled to gels ⁽¹⁾.
B. Inhibitor
Clot inhibition was tested by incubation for 2 hours at 37°C, and was 62% at 500 μg/mL (highest conc. tested).

The antibody is conformation dependent and is not useful for Western blotting.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany
 

References

1. The production and characterisation of a panel of ten murine monoclonal antibodies to human procoagulant factor VIII. Griffin, B. D., et al. Thrombosis and Haemostasis, 1986; 55: 40-46.
2. Development, optimization and use of an enzyme linked immunosorbent assay (ELISA) to measure factor VIII antigen utilizing monoclonal antibodies. Hornsey, V. S., et al. Transfusion Medicine, 1992; 2: 223-229.
3. In vivo production of human factor VII in mice after intrasplenic implantation of primary fibroblasts transfected by receptor-mediated, adenovirus-augmented gene delivery. Zatloukal, K., et al. Proceeds of the National Academy of Science USA, 1994; 91: 5148-5152.
4. Some factor VIII inhibitor antibodies recognize a common epitope corresponding to C2 domain amino acids 2248 through 2312, which overlap a phospholipid-binding site. Scandella, D., et al. Blood, 1995; 86: 1811-1819.