thrombomodulin, rabbit lung, 30 units, 1.0 mL vial

The IMUBIND( Thrombomodulin (TM) ELISA is a "sandwich" ELISA employing a monoclonal antibody which recognizes the EGF1 - EGF2 domains of TM. Specificity of the capture antibody for native, complexed and truncated TM was confirmed by Western Blot analysis. Samples incubate in microtest wells precoated with the capture antibody. A second horseradish peroxidase (HRP) conjugated monoclonal antibody specific for the EGF5 - EGF6 domains recognizes the bound TM, completing the antibody-antigen-antibody "sandwich".After addition of a peroxidase enzyme substrate (TMB) the colour development is stopped and the intensity of the colour is measured at 450 nm.