murine MAb against human tissue factor

Description
Tissue Factor (TF, CD142) is a 45 kDa transmembrane cell surface glycoprotein known for its role in initiating coagulation. It is comprised of three domains: an extracellular domain (aa 1-219), a hydrophilic spanning domain (aa 220-242) and a cytoplasmic tail (aa 243-263).

Properties
The monoclonal antibody ADG4507 (clone VIC7, subclass IgG₁) recognizes an epitope within amino acids 203-214 of human Tissue Factor.⁽¹⁾
Mice were immunized with purified apoprotein of human tissue factor (MW = 47 kDa). The antibody has been purified from cell culture supernatant using Protein A affinity chromatography.
The antibody neutralizes brain and placental thrombo-plastin.

Presentation
Screw capped vial containing 0.5 mg of purified antibody in PBS pH 7.4, 0.01 % ProClin300. The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
ELISA
The antibody can be used as capture antibody in ELISAs.^{(1, 3)}
Western Blot Analysis
The antibody can be used in Western blot analysis, detecting human tissue factor under reducing and non-reducing conditions at a working concentration of 2-10 μg/mL ⁽⁸⁾


Flow Cytometry
The antibody may be used for analyzing tissue factor expression of a wide variety of cell types including monocytes and HUVEC.^{(2, 4 - 6)}
Immunhistochemistry
The antibody has been used for immunofluorescence staining and has shown a strong reactivity for tissue factor in frozen tissue sections of both the kidney (glomerular epithelia) and the epidermis (upper cell layer).^{(1, 2, 7)}

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany




References

1. Immunological and functional analyses of the extracellular domain of human tissue factor. Magdolen V et al., Biol Chem 1998, 379: 157-165.
2. Morrissey JH et al., CD142 (tissue factor) Workshop Panel report. In: Leucocyte Typing VI: white cell differentiation antigens. Garland Publishing, Inc., New York, 1997, 742-746.
3. Constitutive tissue factor expression of human breast cancer cell line MCF-7 is modulated by growth factors. Flössel C et al., Eur J Cancer. 1992;28A(12):1999-2002.
4. The importance of platelets in the expression of monocyte tissue factor antigen measured by a new whole blood flow cytometric assay. Amirkhosravi A et al., Thromb Haemost 1996, 75: 87-95.
5. Tissue factor expression on monocyte subpopulations during normal pregnancy. Holmes VA et al., Thromb Haemost 2002, 87: 953-958.
6. Flow cytometric analysis of tissue factor (TF) expression on stimulated monocytes-comparison to procoagulant activity of mononuclear blood cells. Luther T et al., Blut 1990, 61: 375-378.
7. Localization of Tissue Factor in Actin-Filament-Rich Membrane Areas of Epithelial Cells. Müller M et al., Exp Cell Res 1999, 248:136-147.
8. Role of tissue factor in adhesion of mononuclear phagocytes to and trafficking through endothelium in vitro. Randolph GJ et al., Blood. 1998 Dec 1;92(11):4167-4177.