mAb against human Vitronectin, 250 microgram, liquid

The Vitronectin (VN) ELISA is a "sandwich" ELISA employing a monoclonal and a polyclonal antibody specific for human Vitronectin.Diluted plasma samples are added to microwells coated with the monoclonal antibody against Vitronectin. During an incubation period, Vitronectin present in the sample will bind to the antibody coated to the wells. Following a washing step, a horseradish peroxidase (HRP) conjugated rabbit anti-Vitronectin polyclonal antibody is added to the microwells and binds to the Vitronectin protein captured on the plate during a short incubation period. Following another washing step, the addition of a TMB substrate and its subsequent reaction with the HRP present generates a blue colored solution. The reaction is stopped by the adding sulfuric acid, which turns the solution color yellow. Measuring the solution absorbance at 450 nm and extrapolating the value with those of a standard curve determines the level of Vitronectin in the diluted plasma sample.