3-Methoxytyramine Plasma RIA FastTrack (3-MT RIA)

Advantages:-

• New biomarker of phaeochromocytoma and paraganglioma tumours
• The first commercial assay worldwide!
• Speed and ease of use
• 48 duplicate determiantions or 96 single
• Easy sample preparation (extraction)
• No known interferences by medical drugs
• High correlation with LCMS-MS
• Wide standard ranges, conventional measurement of pathological samples without predilution

Cells derived form neuroendocrine tumours (e.g. pheochromcytoma) are known to produce catecholamines which are secreted
episodically via vesicles into the bloodstream.

But beside this a small portion of the catecholamines is metabolized inside the cells to the corresponding catecholamines
metabolites – namely metanephrine, normetanephrine and 3-methoxytyramine (3-MT) – which are secreted at low levels
continuously into the bloodstream.

3-methoxytyramine is a metabolite of the neurotransmitter Dopamine formed by the introduction of a methyl group to dopamine by
the enzyme catechol-O-methyl transferase (COMT).

The measurement of 3-MT allows the detection of dopamine-producing tumours: For example a substantial number of patients
with head and neck paragangliomas (HNPGL) have biochemically active tumours and only display increased excretion of 3-methoxytyramine, but not of other catecholamines or their metabolites.

The combined levels of free metanephrine/normetanephrine and free 3-MT in plasma indicate a higher number of biochemically
active HNPGLs than the 24-h urinary excretion rates of these markers.

In addition 3-MT acts as a neuromodulator and can also induce behavioral effects involved in movement control in a dopamineindependent manner. Most of these effects are mediated by the trace amine associated receptor 1 (TAAR1) which in turn is
involved in abnormal dopaminergic transmission, such as Parkinson's disease, dyskinesia and schizophrenia.

th head-and-neck paragangliomas (HNPGL) have biochemically active tumors and only display increased excretion of 3-
methoxytyramine, but not of other catecholamines or their metabolites.
The combined levels of free metanephrine/normetanephrine and free 3-MT in plasma indicate a higher number of biochemically
active HNPGLs than the 24-h urinary excretion rates of these markers.

In addition 3-MT acts as a neuromodulator and can also induce behavioral effects involved in movement control in a dopamineindependent manner. Most of these effects are mediated by the trace amine associated receptor 1 (TAAR1) which in turn is involved in abnormal dopaminergic transmission, such as Parkinson's disease, dyskinesia and schizophrenia.

• Radioimmunoassay for the fast and quantitative determination in 3-MT in plasma
• Easy sample preparation. Acylation in acylation tubes. 100 tubes per kit. 6 standards, 2 controls, ready for use
• Time: sample preparation 2.5h. RIA 1h - overnight
• ​EDTA / Heparin Plasma samples