Enzyme Immunoassay for the quantitative determination of adrenaline (epinephrine) and noradrenaline (norepinephrine) in plasma. Adrenaline (epinephrine) and noradrenaline (norepinephrine) are extracted from a plasma sample* ) by using a cis-diol-specific affinity gel, acylated and then modified enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. NAME: 2-CAT Plasma ELISA High SensitiveDESCRIPTION: Enzyme immunoassay (ELISA) for the high-sensitive quantitative determination of Adrenaline (Epinephrine) and Noradrenaline (Norepinephrine) in plasmaDESIGN: Common extraction of plasma in 48-well macrotiter plates. Acylation in liquid phase. 6 standards, 2 controls, ready for use.SAMPLE VOLUME: 100-600 µl plasmaTOTAL ASSAY TIME: Extraction, acylation and enzymatic conversion 4 hours and ELISA overnightSTANDARD RANGE: Adrenaline 0 / 20 - 3200 pg/ml;Noradrenaline 0 / 80 - 12800 pg/mlSENSITIVITY: Adrenaline: 7 pg/ml; Noradrenaline: 35 pg/mlSTORAGE: 2 - 8 °CKIT: 2 x 96 DeterminationsADVANTAGES: Determine both analytes with one kitNo known interference by medical drugsWide standard ranges. convenient measurement of pathological samples without predilutionHigh correlation with HPLCCE MARK: CE Marked
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