125I – Radioimmunoassay for the quantitative determination of Serotonin in serum, urine, and platelets.For research use only (RUO) this kit can also be used for cerebrospinal fluid (CSF) and platelet-free plasma (PFP). Please refer to appendix 1. First, Serotonin is quantitatively acylated. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. After centrifugation and decantation of the supernatant the precipitate is counted in a gamma counter. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample.Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards. NAME: Serotonin RIA Fast TrackDESCRIPTION: Radioimmunoassay (RIA) for the quantitative determination of SerotoninDESIGN: Common sample preparation for all body fluids. Acylation in liquid phase. 6 standards, 2 controls, ready for use. Double antibody technique.SAMPLE VOLUME: 25 µl serum, urine, plateletsTOTAL ASSAY TIME: Acylation 30 min and RIA 2 hSTANDARD RANGE: 0 / 15 - 2 500 ng/mlSENSITIVITY: 6.7 ng/ml serum, urine, platelets, 0.3 ng/ml CSF, PFPSTORAGE: 2 - 8 °CKIT: 96 DeterminationsADVANTAGES: Total assay time approx. 2.5 hoursNo interference by medical drugs knownWide standard ranges. convenient measurement of pathological samples without predilutionGood correlation to the other commer15 week shelf-lifeCE MARK: CE Marked
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