Enzyme Immunoassay for the quantitative determination of Metanephrine and Normetanephrine in urine. During the sample preparation Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. NAME: 2-MET Urine ELISA Fast TrackDESCRIPTION: Fast Track enzyme immunoassay (ELISA) for the quantitative determination of Metanephrine and Normetanephrine in urineDESIGN: Common sample preparation. Acylation in liquid phase. 6 standards, 2 controls, ready for use.SAMPLE VOLUME: 25 µl urineTOTAL ASSAY TIME: Hydrolysis and acylation 45 min and ELISA 1 hSTANDARD RANGE: Metanephrine 0 / 20 - 2 000 ng/mlNormetanephrine 0 / 30 - 3 000 ng/mlSENSITIVITY: Metanephrine 13 ng/mlNormetanephrine 23 ng/mlSTORAGE: 2 - 8 °CKIT: 2 x 96 DeterminationsADVANTAGES: Total assay time approx. 1h 45 minNo interference by medical drugs knownWide standard ranges. convenient measurement of pathological samples without predilutionGood correlation with HPLC (UK NEQAS)CE MARK: CE Marked
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