The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a standard curve prepared with known standards. Metanephrine Urine RIA Fast TrackDESCRIPTION: Fast Track Radioimmunoassay (RIA) for the quantitative determination of Metanephrine in urineDESIGN: Common sample preparation. Acylation in liquid phase. 6 standards, 2 controls, ready for use. Double antibody technique.SAMPLE VOLUME: 25 µl urineTOTAL ASSAY TIME: Hydrolysis and acylation 45 min and RIA 1.5 hSTANDARD RANGE: 0 / 20 - 2 000 ng/mlSENSITIVITY: 8 ng/mlSTORAGE: 2 - 8 °CKIT: 100 DeterminationsADVANTAGES: Total assay time approx. 2h 15 minNo interference by medical drugs knownWide standard ranges. convenient measurement of pathological samples without predilutionGood correlation with HPLC (UK NEQAS)15 week shelf-life
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