125I – Radioimmunoassay for the quantitative determination of free Metanephrine in plasma. First, the plasma proteins are removed by precipitation. Then the metanephrine (metadrenaline) is acylated. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards. NAME: Metanephrine Plasma RIA Fast TrackDESCRIPTION: Fast Track Radioimmunoassay (RIA) for the quantitative determination of Free Metanephrine in plasmaDESIGN: Common sample preparation. Acylation in liquid phase. 6 standards, 2 controls, ready for use. Double antibody technique.SAMPLE VOLUME: 500 µl plasmaTOTAL ASSAY TIME: Sample preparation 15 min and RIA 3 h 45 min (or overnight)STANDARD RANGE: 0 / 36 - 3 600pg/mlSENSITIVITY: 19 pg/mlSTORAGE: 2 - 8 °CKIT: 100 DeterminationsADVANTAGES: Simple sample preparationWide standard ranges. convenient measurement of pathological samples without predilutionSpecific for the biologically relevant L-metanephrine15 week shelf-lifeCE MARK: CE Marked
Academic Discount – Customers performing Academic Research are entitled to a 10% discount on all Coagulation and Biochemistry products. Use Voucher Code ACAD10 at checkout.