3-CAT RIAFast Track (Adrenaline/Noradrenaline/Dopamine)

3-CAT RIAFast Track (Adrenaline/Noradrenaline/Dopamine)

Brand:LDN
Product Code:BA R-6600
Price: £825.00

125I – Radioimmunoassay for the quantitative determination of Adrenaline (Epinephrine), Noradrenaline (Norepinephrine) and Dopamine in plasma and urine. Adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are extracted by using a cis-diolspecific affinity gel, acylated and then converted enzymatically. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a standard curve prepared with known standards.NAME: 3-CAT RIA Fast TrackDESCRIPTION: Fast Track Radioimmunoassay (RIA) for the quantitative determination of of Adrenaline (Epinephrine), Noradrenaline (Norepinephrine), and Dopamine in plasma and urineDESIGN: Extraction and Acylation in 48-well microtiter plates (PATENT EPA 1835 290). 6 standards, 2 controls, ready for use. Double antibody technique.SAMPLE VOLUME: 10 µl urine, 300 µl plasmaTOTAL ASSAY TIME: Extraction and acylation 65 min and RIA 1.5 hSTANDARD RANGE: Adrenaline 0 / 1 - 200 ng/mlNoradrenaline 0 / 5 - 1 000 ng/mlDopamine 0 / 4.5 - 2 000 ng/mlSENSITIVITY: Adrenaline: 0.3 ng/ml urine, 10 pg/ml plasmaNoradrenaline: 1.5 ng/ml urine, 50 pg/ml plasma Dopamine: 4.5 ng/ml urine, 20 pg/ml plasmaSTORAGE: 2 - 8 °CKIT: 3 x 96 DeterminationsADVANTAGES: Total assay approximately 2.5 hoursDetermine all three analytes with one kitNo known interference by medical drugsWide standard ranges. convenient measurement of pathological samples without predilutionHigh correlation with HPLC15 week shelf-lifeCE MARKed


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