125I – Radioimmunoassay for the quantitative determination of Adrenaline (Epinephrine) and Noradrenaline (Norepinephrine) in plasma and urine. Adrenaline (epinephrine) and noradrenaline (norepinephrine) are extracted by using a cis-diol- specific affinity gel, acylated and then converted enzymatically. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.NAME: 2-CAT RIA Fast TrackDESCRIPTION: Fast Track Radioimmunoassay (RIA) for the quantitative determination of Adrenaline (Epinephrine) and Noradrenaline (Norepinephrine) in plasma and urineDESIGN: Extraction and Acylation in 48-well microtiter plates (PATENT EPA 1835 290). 6 standards, 2 controls, ready for use. Double antibody technique.SAMPLE VOLUME: 10 µl urine, 300 µl plasmaTOTAL ASSAY TIME: Extraction and acylation 65 min and RIA 1.5 hSTANDARD RANGE: Adrenaline 0 / 1 - 200 ng/mlNoradrenaline 0 / 5 - 1 000 ng/mlSENSITIVITY: Adrenaline: 0.3ng/ml urine, 10 pg/ml plasmaNoradrenaline: 1.5 ng/ml urine, 50 pg/ml plasmaSTORAGE: 2 - 8 °CKIT: 2 x 96 DeterminationsADVANTAGES: Total assay approximately 2.5 hoursDetermine both analytes with one kitNo known interference by medical drugsWide standard ranges. convenient measurement of pathological samples without predilutionHigh correlation with HPLC15 week shelf-lifeCE MARKed
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