125I – Radioimmunoassay for the quantitative determination of noradrenaline (norepinephrine) in plasma and urine. Noradrenaline (norepinephrine) is extracted by using a cis-diol- specific affinity gel, acylated and then converted enzymatically. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a standard curve prepared with known standards.
NAME: Noradrenaline RIA Fast Track
DESCRIPTION: Fast Track Radioimmunoassay (RIA) for the quantitative determination of Noradrenaline (Norepinephrine) in plasma and urine
DESIGN: Extraction and Acylation in 48-well microtiter plates (PATENT EPA 1835 290). 6 standards, 2 controls, ready for use. Double antibody technique.
SAMPLE VOLUME: 10 µl urine, 300 µl plasma
TOTAL ASSAY TIME: Extraction and acylation 65 min and RIA 1.5 h
STANDARD RANGE: 0 / 5 - 1 000 ng/ml
SENSITIVITY: 1.5 ng/ml urine, 50 pg/ml plasma
STORAGE: 2 - 8 °CKIT: 96 Determinations
ADVANTAGES: Total assay approximately 2.5 hours
No known interference by medical drugs
Wide standard ranges. convenient measurement of pathological samples without predilution
High correlation with HPLC15 week shelf-life
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