Noradrenaline RIAFast Track

Noradrenaline RIAFast Track

Brand:LDN
Product Code:BA R-6200
Price: £412.00

125I – Radioimmunoassay for the quantitative determination of noradrenaline (norepinephrine) in plasma and urine. Noradrenaline (norepinephrine) is extracted by using a cis-diol- specific affinity gel, acylated and then converted enzymatically. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a standard curve prepared with known standards.

NAME: Noradrenaline RIA Fast Track

DESCRIPTION: Fast Track Radioimmunoassay (RIA) for the quantitative determination of Noradrenaline (Norepinephrine) in plasma and urine

DESIGN: Extraction and Acylation in 48-well microtiter plates (PATENT EPA 1835 290). 6 standards, 2 controls, ready for use. Double antibody technique.

SAMPLE VOLUME: 10 µl urine, 300 µl plasma

TOTAL ASSAY TIME: Extraction and acylation 65 min and RIA 1.5 h

STANDARD RANGE: 0 / 5 - 1 000 ng/ml

SENSITIVITY: 1.5 ng/ml urine, 50 pg/ml plasma

STORAGE: 2 - 8 °CKIT: 96 Determinations

ADVANTAGES: Total assay approximately 2.5 hours

No known interference by medical drugs

Wide standard ranges. convenient measurement of pathological samples without predilution

High correlation with HPLC15 week shelf-life

CE MARKed


Academic Discount – Customers performing Academic Research are entitled to a 10% discount on all Coagulation and Biochemistry products. Use Voucher Code ACAD10 at checkout.
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