125I – Radioimmunoassay for the direct quantitative determination of melatonin in saliva.The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. After centrifugation and decantation of the supernatant the precipitate is counted in a gamma counter. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards. NAME: Melatonin Saliva Direct RIADESCRIPTION: Radioimmunoassay (RIA) for the quantitative determination of of Melatonin in salivaDESIGN: Common sample preparation. 6 standards, 2 controls, ready for use. Double antibody technique.SAMPLE VOLUME: 500 µl salivaTOTAL ASSAY TIME: Sample preparation 1 hour and RIA 20-24 hoursSTANDARD RANGE: 0 / 3 - 300 pg/mlSENSITIVITY: 1.4 pg/mlSTORAGE: 2 - 8 °CKIT: 100 DeterminationsADVANTAGES: Direct assayTest for Melatonin in salivaHigh specificity and sensitivity15 week shelf-lifeReady for use tracersCE MARK: CE Marked
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