125I – Radioimmunoassay for the direct quantitative determination of melatonin in human serum and plasma. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. After centrifugation and decantation of the supernatant the precipitate is counted in a gamma counter. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.NAME: Melatonin Direct RIADESCRIPTION: Radioimmunoassay (RIA) for the quantitative determination of of Melatonin in serum and plasmaDESIGN: Common sample preparation. 7 standards, 2 controls, ready for use. Double antibody technique.SAMPLE VOLUME: 150 µl serum or plasmaTOTAL ASSAY TIME: Sample preparation 1 hour and RIA 20-24 hoursSTANDARD RANGE: 0 / 3 - 1 000 pg/mlSENSITIVITY: 2,3 pg/mlSTORAGE: 2 - 8 °CKIT: 100 DeterminationsADVANTAGES: Direct assayHigh specificity and sensitivity15 week shelf-lifeReady for use tracersCE MARKed
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