Immunoassay for the detection of IgG or IgM antibodies against Parvovirus B19 specific antigens in human serum. The Parvovirus B19 ViraStripe® IgG, IgM is an immunoblot which carries highly purified Parvovirus B19 specific antigens: VP1, VP1-N, VP1-N, VP2-Cs, VP2-Cw, VP2 and NS1. The antigens VP1, VP1-N, VP2-Cs, VP2-Cw and NS1 exhibit denatured protein structures presenting linear epitopes. VP1-N and VP2 are made of soluble particles presenting conformational epitopes. The assay is intented for the confirmation of Parvovirus B19 infections and for assessment of the antibody status. Combined analysis of samples with Parvovirus B19 ViraStripe® IgG and IgM provides supportive evidence on the putative time point of infection and the corresponding phase of infection by differentiating acute, recent and past infections. The control section of each strip includes a serum control, three conjugate controls and a cut off control.