murine MAb against human tissue factor

Description
Tissue Factor (TF, CD142) is a 45 kDa transmembrane cell surface glycoprotein known for its role in initiating coagulation. It is comprised of three domains: an extracellular domain (aa 1-219), a hydrophilic spanning domain (aa 220-242) and a cytoplasmic tail (aa 243-263).

Properties
The monoclonal antibody ADG4508 (clone VD8, subclass IgG1) is directed against an epitope within aa 1-25, the extracellular domain of human tissue factor.⁽¹⁾
Mice were immunized with purified apoprotein of human tissue factor (MW = 47 kDa). The antibody has been purified from cell culture supernatant using Protein A affinity chromatography.
The antibody reacts with and neutralizes the purified apoprotein of human tissue factor, native human brain and placental thromboplastin, recognize tissue factor on the surface of tumor cells and LPS stimulated monocytes.

Presentation
Clear glass, screw-capped vial containing 0.5 mg of purified IgG lyophilized from a 1 mg/mL solution PBS, pH 7,4 with 100 mM mannitol added as an excipient.

Reconstitution
Add 0.5 mL of filtered deionized or sterile water to the vial to generate a 1 mg/mL stock solution.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
Immunohistochemistry
The antibody is recommended for staining both cryo- and formalin-fixed, paraffin-embedded human sections Concentrations ranging from 1-10 μg/mL using incubations of 1 hour at 18°-25°C or overnight at 4°C have been used.
Western Blot Analysis
The antibody is suitable for Western blot analysis, detecting 50 ng of human tissue factor under reducing and non-reducing conditions at a working concentration of 5-10 μg/mL.
Flow Cytometry
The antibody is suitable for flow cytometric staining.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany



References

1. Immunological and functional analyses of the extracellular domain of human tissue factor. Magdolen V et al., Biol Chem 1998, 379: 157-165.
2. Expression, Localization, and Activity of Tissue Factor Pathway Inhibitor in Normal and Atherosclerotic Human Vessels. Crawley, J., et al., Arteriosclerosis, Thrombosis and Vascular Biology 2000, 20: 1362-1373.
3. Expression of Tissue Factor in High-Grade Carotid Artery Stenosis. Jander, S., et al., Stroke 2001, 32: 850-854.
4. Increased Tissue Factor Expression Predicts Development of Cardiac Allograft Vasculopathy. Yen, M. H., et al. Circulation 2002, 106: 1379-1383.
5. Human Pancreatic Duct Cells Exert Tissue Factor-Dependent Procoagulant Activity, Relevance to Islet Transplantation. Beuneu, C., et al. Diabetes 2004, 53: 1407-1411.
6. In Situ detection of tissue factor in vascular endothelial cells: Correlation with the malignant phenotype of human breast disease. Contrino, J., et al. Nature Medicine 1996, 2: 209-215.
7. Flow cytometric analysis of tissue factor (TF) expression on stimulated monocytes--comparison to procoagulant activity of mononuclear blood cells. Luther T et al., Blut. 1990 Dec;61(6):375-378.
8. Tissue factor-dependent pathway is not involved in exercise-induced formation of thrombin and fibrin. Weiss C et al., J Appl Physiol. 2002 Jan;92(1):211-218.