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The specific activity of the human Lys-plasmin is approximately 7.5 units/mg. Human Lys-plasmin prepared from human glu-plasminogen (purified from fresh frozen human plasma) by rapid activation using matrix bound urokinase and purified using a lysine-Sepharose column.
Bovine plasminogen is activated by urokinase (uPA), tissue plasminogen activation (tPA), and streptokinase-human plasminogen/plasmin complex. Bovine plasminogen is not activated by streptokinase alone. tPA activation is stimulated several hundredfold by poly D-lysine, CNBr-fibrinogen, and des AA-fib..
The IMUBIND( Thrombomodulin (TM) ELISA is a "sandwich" ELISA employing a monoclonal antibody which recognizes the EGF1 - EGF2 domains of TM. Specificity of the capture antibody for native, complexed and truncated TM was confirmed by Western Blot analysis. Samples incubate in microtest wells precoate..